Assessing the Diversity of Marine Bacterial β-Glucosidases by Capillary Electrophoresis Zymography
- Author(s)
- Jesús M. Arrieta, Gerhard J. Herndl
- Abstract
We propose a new method for the fast separation and detection of β-glucosidases in environmental samples. With this approach, β-glucosidases extracted from bacteria are evidenced by substrate-incorporated capillary electrophoresis (CE zymography) and their kinetic parameters can be determined by repeated injections using different substrate concentrations. Preliminary results obtained with natural bacterial communities from the coastal North Sea suggest that the diversity of β-glucosidases in the marine environment might be much higher than previously observed.
- Organisation(s)
- Functional and Evolutionary Ecology
- External organisation(s)
- Royal Netherlands Institute for Sea Research
- Journal
- Applied and Environmental Microbiology
- Volume
- 67
- Pages
- 4896-4900
- No. of pages
- 5
- ISSN
- 0099-2240
- DOI
- https://doi.org/10.1128/AEM.67.10.4896-4900.2001
- Publication date
- 10-2001
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106021 Marine biology
- ASJC Scopus subject areas
- Biotechnology, Food Science, Applied Microbiology and Biotechnology, Ecology
- Sustainable Development Goals
- SDG 14 - Life Below Water
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/assessing-the-diversity-of-marine-bacterial-glucosidases-by-capillary-electrophoresis-zymography(c4e6374a-939e-4100-b3fe-76e08d4083e5).html