Quantification of aquatic viruses by flow cytometry

Author(s)

Corina P. D. Brussaard, Jerome P. Payet, Christian Winter, Markus Weinbauer

Abstract

For many laboratories, flow cytometry is becoming the routine method for quantifying viruses in aquatic systems

because of its high reproducibility, high sample throughput, and ability to distinguish several subpopulations

of viruses. Comparison of viral counts between flow cytometry and epifluorescence microscopy typically

shows slopes that are statistically not distinguishable from 1, thus confirming the usefulness of flow cytometry.

Here we describe in detail all steps in the procedure, discuss potential problems, and offer solutions.

Organisation(s)

Functional and Evolutionary Ecology

External organisation(s)

Royal Netherlands Institute for Sea Research, University of British Columbia (UBC), Laboratoire d'Océanographie de Villefranche

Pages

102-109

No. of pages

Publication date

2010

Austrian Fields of Science 2012

106022 Microbiology

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