Microbes mediating the sulfur cycle in the Atlantic Ocean and their link to chemolithoautotrophy

Author(s)
Daniele De Corte, Simone Muck, Johanna Tiroch, Catalina Mena, Gerhard J Herndl, Eva Sintes
Abstract

Only about 10%-30% of the organic matter produced in the epipelagic layers reaches the dark ocean. Under these limiting conditions, reduced inorganic substrates might be used as an energy source to fuel prokaryotic chemoautotrophic and/or mixotrophic activity. The aprA gene encodes the alpha subunit of the adenosine-5 '-phosphosulfate (APS) reductase, present in sulfate-reducing (SRP) and sulfur-oxidizing prokaryotes (SOP). The sulfur-oxidizing pathway can be coupled to inorganic carbon fixation via the Calvin-Benson-Bassham cycle. The abundances of aprA and cbbM, encoding RuBisCO form II (the key CO2 fixing enzyme), were determined over the entire water column along a latitudinal transect in the Atlantic from 64 degrees N to 50 degrees S covering six oceanic provinces. The abundance of aprA and cbbM genes significantly increased with depth reaching the highest abundances in meso- and upper bathypelagic layers. The contribution of cells containing these genes also increased from mesotrophic towards oligotrophic provinces, suggesting that under nutrient limiting conditions alternative energy sources are advantageous. However, the aprA/cbbM ratios indicated that only a fraction of the SOP is associated with inorganic carbon fixation. The aprA harbouring prokaryotic community was dominated by Pelagibacterales in surface and mesopelagic waters, while Candidatus Thioglobus, Chromatiales and the Deltaproteobacterium_SCGC dominated the bathypelagic realm. Noticeably, the contribution of the SRP to the prokaryotic community harbouring aprA gene was low, suggesting a major utilization of inorganic sulfur compounds either as an energy source (occasionally coupled with inorganic carbon fixation) or in biosynthesis pathways.

Organisation(s)
Functional and Evolutionary Ecology, Department of Physiological Chemistry
External organisation(s)
Carl von Ossietzky Universität Oldenburg, Instituto Español de Oceanografía, Utrecht University, University of Vienna
Journal
Environmental Microbiology
Volume
23
Pages
7152-7167
No. of pages
16
ISSN
1462-2912
DOI
https://doi.org/10.1111/1462-2920.15759
Publication date
11-2021
Peer reviewed
Yes
Austrian Fields of Science 2012
106021 Marine biology
Keywords
ASJC Scopus subject areas
Ecology, Evolution, Behavior and Systematics, Microbiology
Sustainable Development Goals
SDG 14 - Life Below Water
Portal url
https://ucrisportal.univie.ac.at/en/publications/631f8922-2a75-4d14-9cbd-6211b489b3ac