Development and deployment of a point-source digital inline holographic microscope for the study of plankton and particles to a depth of 6000 m

Author(s)
Alexander B. Bochdansky, Manfred H. Jericho, Gerhard J. Herndl
Abstract

A point-source digital inline holographic microscope (DIHM) was designed for the imaging of particles from 50 μm to several millimeters in size. The DIHM operates autonomously without connection to external recording devices or power sources and delivers 4.2 megapixel images at a rate of approximately 7 images s-1, each image representing a snapshot of 1.8 mL seawater. Reliance on largely off-the-shelf components, and simplification in its construction makes this camera system adaptable to various particle size ranges and environments, and easy-to-operate for nonexpert users. The DIHM produced sharp images of protists with skeletal structures (e.g., acantharians, tintinnids, dinoflagellates), mesoplankton (e.g., copepods, appendicularians, medusae), Trichodesmium colonies and marine snow particles while descending in the water column at 1 m s-1, a typical velocity for deployments of tethered instruments and samplers from oceanographic vessels. To validate the usefulness of the new instrument in an oceanographic context, data are presented of the surface distribution of Trichodesmium spp., and of the vertical frequency distribution of fecal pellets and other particles in the deep sea. The point-source DIHM has the potential to become a standard instrument on the CTD rosette (i.e., on the basic oceanographic instrument and sampling frame) in the future providing a permanent archival record of the water column that can be mined for specific target particles in the future.

Organisation(s)
External organisation(s)
Royal Netherlands Institute for Sea Research, Old Dominion University, Dalhousie University
Journal
Limnology and Oceanography-Methods
Volume
11
Pages
28-40
No. of pages
13
ISSN
1541-5856
DOI
https://doi.org/10.4319/lom.2013.11.28
Publication date
01-2013
Peer reviewed
Yes
Austrian Fields of Science 2012
106021 Marine biology
Keywords
ASJC Scopus subject areas
Ocean Engineering
Portal url
https://ucris.univie.ac.at/portal/en/publications/development-and-deployment-of-a-pointsource-digital-inline-holographic-microscope-for-the-study-of-plankton-and-particles-to-a-depth-of-6000-m(81653500-8906-4a9b-bec6-7e7d62971e79).html