Ammonia-oxidizing archaea possess a wide range of cellular ammonia affinities

Author(s)
Man-Young Jung, Christopher J. Sedlacek, K. Dimitri Kits, Anna J. Mueller, Sung-Keun Rhee, Linda Hink, Graeme W. Nicol, Barbara Bayer, Laura Lehtovirta-Morley, Chloe Wright, Jose R. de la Torre, Craig W. Herbold, Petra Pjevac, Holger Daims, Michael Wagner
Abstract

Nitrification, the oxidation of ammonia to nitrate, is an essential process in the biogeochemical nitrogen cycle. The first step of nitrification, ammonia oxidation, is performed by three, often co-occurring guilds of chemolithoautotrophs: ammonia-oxidizing bacteria (AOB), archaea (AOA), and complete ammonia oxidizers (comammox). Substrate kinetics are considered to be a major niche-differentiating factor between these guilds, but few AOA strains have been kinetically characterized. Here, the ammonia oxidation kinetic properties of 12 AOA representing all major cultivated phylogenetic lineages were determined using microrespirometry. Members of the genus Nitrosocosmicus have the lowest affinity for both ammonia and total ammonium of any characterized AOA, and these values are similar to previously determined ammonia and total ammonium affinities of AOB. This contrasts previous assumptions that all AOA possess much higher substrate affinities than their comammox or AOB counterparts. The substrate affinity of ammonia oxidizers correlated with their cell surface area to volume ratios. In addition, kinetic measurements across a range of pH values supports the hypothesis that-like for AOB-ammonia and not ammonium is the substrate for the ammonia monooxygenase enzyme of AOA and comammox. Together, these data will facilitate predictions and interpretation of ammonia oxidizer community structures and provide a robust basis for establishing testable hypotheses on competition between AOB, AOA, and comammox.

Organisation(s)
Centre for Microbiology and Environmental Systems Science, Department of Microbiology and Ecosystem Science, The Comammox Research Platform, Department of Evolutionary Biology, Department of Inorganic Chemistry
External organisation(s)
San Francisco State University, Jeju National University, Chungbuk National University (CBNU), Université de Lyon, University of East Anglia, Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna, Vienna, Austria, Aalborg University (AAU)
Journal
ISME Journal
No. of pages
12
ISSN
1751-7362
DOI
https://doi.org/10.1038/s41396-021-01064-z
Publication date
07-2021
Peer reviewed
Yes
Austrian Fields of Science 2012
106022 Microbiology
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/ammoniaoxidizing-archaea-possess-a-wide-range-of-cellular-ammonia-affinities(6d0727fb-f434-441c-a2df-cdcc3af388e0).html