Singe cell genomes of Prochlorococcus, Synechococcus, and sympatric microbes from diverse marine environments

Author(s)
Paul M. Berube, Steven J. Biller, Thomas Hackl, Shane L. Hogle, Brandon M. Satinsky, Jamie W. Becker, Rogier Braakman, Sara B. Collins, Libusha Kelly, Jessie Berta-Thompson, Allison Coe, Kristin Bergauer, Heather A. Bouman, Thomas J. Browning, Daniele De Corte, Christel Hassler, Yotam Hulata, Jeremy E. Jacquot, Elizabeth W. Maas, Thomas Reinthaler, Eva Sintes, Taichi Yokokawa, Debbie Lindell, Ramunas Stepanauskas, Sallie W. Chisholm
Abstract

Prochlorococcus and Synechococcus are the dominant primary producers in marine ecosystems and perform a significant fraction of ocean carbon fixation. These cyanobacteria interact with a diverse microbial community that coexists with them. Comparative genomics of cultivated isolates has helped address questions regarding patterns of evolution and diversity among microbes, but the fraction that can be cultivated is miniscule compared to the diversity in the wild. To further probe the diversity of these groups and extend the utility of reference sequence databases, we report a data set of single cell genomes for 489 Prochlorococcus, 50 Synechococcus, 9 extracellular virus particles, and 190 additional microorganisms from a diverse range of bacterial, archaeal, and viral groups. Many of these uncultivated single cell genomes are derived from samples obtained on GEOTRACES cruises and at well-studied oceanographic stations, each with extensive suites of physical, chemical, and biological measurements. The genomic data reported here greatly increases the number of available Prochlorococcus genomes and will facilitate studies on evolutionary biology, microbial ecology, and biological oceanography.

Organisation(s)
Department of Limnology and Bio-Oceanography
External organisation(s)
Massachusetts Institute of Technology, Wellesley College, Yeshiva University, University of Oxford, GEOMAR Helmholtz-Zentrum für Ozeanforschung, Japan Agency for Marine-Earth Science and Technology, Université de Genève, Technion - Israel Institute of Technology, University of Southern California, Ministry For Primary Industries, Bigelow Laboratory for Ocean Sciences
Journal
Scientific Data
Volume
5
No. of pages
11
ISSN
2052-4463
DOI
https://doi.org/10.1038/sdata.2018.154
Publication date
09-2018
Peer reviewed
Yes
Austrian Fields of Science 2012
Marine biology, Microbiology
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/singe-cell-genomes-of-prochlorococcus-synechococcus-and-sympatric-microbes-from-diverse-marine-environments(035e66b2-3a9c-4593-a5f0-1c8317be846c).html