Ultrastructure of marine snow. I. Transmission electron microscopy methodology
- Author(s)
- Gary G. Leppard, Andreas Heissenberger, Gerhard J. Herndl
- Abstract
Four different approaches to determine the ultrastructure of amorphous aggregates and their principal components are evaluated for their ability to resolve structural components as small as 1 nm. Based on extensive comparative analysis, the advantages and major drawbacks of the different methods are discussed. Based on experience with marine snow from the Adriatic Sea, it is concluded that only a combination of fixation and preservation treatments ensures optimal interpretation of the results. Specific emphasis is placed on the use of a hydrophilic resin (Nanoplast®) which allows fixation of highly hydrated polysaccharide fibrils in their original configuration and subsequent visualization on a nm scale.
- Organisation(s)
- External organisation(s)
- Environment and Climate Change Canada, McMaster University, Universität Wien
- Journal
- Marine Ecology Progress Series
- Volume
- 135
- Pages
- 289-298
- No. of pages
- 10
- ISSN
- 0171-8630
- DOI
- https://doi.org/10.3354/meps135289
- Publication date
- 05-1996
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106021 Marine biology
- Keywords
- ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics, Aquatic Science, Ecology
- Sustainable Development Goals
- SDG 14 - Life Below Water
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/ultrastructure-of-marine-snow-i-transmission-electron-microscopy-methodology(d5b1cc79-9d88-4aee-8617-bb026a5e0857).html