Quantifying substrate uptake by individual cells of marine bacterioplankton by catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography

Author(s)

Eva Sintes, Gerhard J. Herndl

Abstract

Catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO-CARD-FISH) is increasingly being used to obtain qualitative information on substrate uptake by individual members of specific prokaryotic communities. Here we evaluated the potential for using this approach quantitatively by relating the measured silver grain area around cells taking up ³H-labeled leucine to bulk leucine uptake measurements. The increase in the silver grain area over time around leucine-assimilating cells of coastal bacterial assemblages was linear during 4 to 6 h of incubation. By establishing standardized conditions for specific activity levels and concomitantly performing uptake measurements with the bulk community, MICRO-CAMD-FISH can be used quantitatively to determine uptake rates on a single-cell level. Therefore, this approach allows comparisons of single-cell activities for bacterial communities obtained from different sites or growing under different ecological conditions.

Organisation(s)

External organisation(s)

Royal Netherlands Institute for Sea Research

Journal

Applied and Environmental Microbiology

Volume

72

Pages

7022-7028

No. of pages

7

ISSN

0099-2240

DOI

https://doi.org/10.1128/AEM.00763-06

Publication date

11-2006

Peer reviewed

Yes

Austrian Fields of Science 2012

106021 Marine biology

ASJC Scopus subject areas

Environmental Science(all), Biotechnology, Microbiology

Sustainable Development Goals

SDG 14 - Life Below Water

Portal url

https://ucris.univie.ac.at/portal/en/publications/quantifying-substrate-uptake-by-individual-cells-of-marine-bacterioplankton-by-catalyzed-reporter-deposition-fluorescence-in-situ-hybridization-combined-with-microautoradiography(d4eb2de8-2ac9-4569-9a63-78cfd48eab11).html